An optimized protocol that improves the quality of rna recovered from lcm samples of mcc zones is illustrated below in terms of specimen freezing cryosectioning staining and dehydration preparation for lcm procedure empirical setting of lcm parameters and performing laser microdissection using ir type of laser arcturus pixcell ii laser capture microdissection system ca usa figure 3. Laser microdissection lmd allows the isolation of individual cells or chromosomes and is a well established technique for sample preparation prior to downstream analysis of the nucleic acid content via pcr or sequencing techniques here we describe the successful combination of the leica lmd system and qiagen kits for purification of nucleic acids even from small amounts. When applied to plant cells and especially to woody tissues lcm requires extensive optimization to overcome such factors as rigid cell walls large central vacuoles intercellular spaces and technical issues with thickness and flatness of the sections.
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